Unit

Content

Time (Hrs)

I

Introduction

·        Importance and relevance to nursing

·        Historical perspective

·        Concepts and terminology

·        Principles of microbiology

3

II

General Characteristics of Microbes

·        Structure and classification of Microbes

·        Morphological types

·        Size and form of bacteria

·        Motility

·        Colonization

·        Growth and nutrition of microbes

·        Temperature

·        Moisture

·        Blood and body fluids

·        Laboratory methods for Identification of Microorganisms

·        Types of Staining – simple, differential (Gram‘s, AFB), special – capsular staining (negative), spore, LPCB, KOH mount.

·        Culture and media preparation – solid and liquid. Types of media – semi synthetic, synthetic, enriched, enrichment, selective and

differential media. Pure culture techniques – tube dilution, pour, spread, streak plate. Anaerobic cultivation of bacteria

10

III

Pathogenic Organisms

·        Micro-organisms: Cocci – gram positive and gram negative; Bacilli – gram positive and gram negative

·        Viruses

·        Fungi: Superficial and Deep mycoses

·        Parasites

·        Rodents & Vectors

·        Characteristics, Source, portal of entry, transmission of infection, Identification of disease producing micro-organisms

4

IV

Immunity

·        Immunity: Types, classification

·        Antigen and antibody reaction

·        Hypersensitivity reactions

·        Serological tests

·        Immuno globulins: Structure, types & properties

·        Vaccines: Types & classification, storage and handling, cold chain, Immunization for various diseases

·        Immunization Schedule

I

HAI (Hospital Acquired Infection)

·         Hospital acquired infection

·         Bundle approach

- Prevention of Urinary Tract Infection (UTI)

2

-  Prevention of Surgical Site Infection (SSI)

-  Prevention of Ventilator Associated events (VAE)

-    Prevention of Central Line Associated Blood Stream Infection (CLABSI)

·         Surveillance of HAI – Infection control team & Infection control committee

II

Isolation Precautions and use of Personal Protective Equipment (PPE)

·         Types of isolation system, standard precaution and transmission- based precautions (Direct Contact, Droplet, Indirect)

·         Epidemiology & Infection prevention – CDC guidelines

·         Effective use of PPE

3

III

Hand Hygiene

·         Types of Hand hygiene.

·         Hand washing and use of alcohol hand rub

·         Moments of Hand Hygiene

·         WHO hand hygiene promotion

1

IV

Disinfection and Sterilization

·         Definitions

·         Types of disinfection and sterilization

·         Environment cleaning

·         Equipment Cleaning

·         Guides on use of disinfectants

·         Spaulding‘s principle

1

V

Specimen Collection (Review)

·         Principle of specimen collection

·         Types of specimens

·         Collection techniques and special considerations

·         Appropriate containers

·         Transportation of the sample

·         Staff precautions in handling specimens

1

VI

BMW (Bio Medical Waste Management)

·        Laundry management process and infection control and prevention

·         Waste management process and infection prevention

·         Staff precautions

·         Laundry management

·         Country ordinance and BMW National guidelines 2017: Segregation of wastes, Colour coded waste containers, waste collection & storage, Packaging & labeling, Transportation

2

VII

Antibiotic Stewardship

·         Importance of Antibiotic Stewardship

·         Anti-Microbial Resistance

·         Prevention of MRSA, MDRO in healthcare setting

2

VIII

Patient Safety Indicators

·         Care of Vulnerable patients

·         Prevention of Iatrogenic injury

·         Care of lines, drains and tubing‘s

·         Restrain policy and care – Physical and Chemical

·         Blood & blood transfusion policy

·         Prevention of IV Complication

·         Prevention of Fall

·         Prevention of DVT

3