Cellular and Molecular Pharmacology M.Pharm First semester

Syllabus

MPL 104T: Cellular and Molecular Pharmacology

Theory                                                                                                                              (60 Hours)

Unit-1: Cell Biology                                                                                                        (12 hours)

Structure and functions of cell and its organelles

Genome organization. Gene expression and its regulation, importance of siRNA and microRNA, gene mapping and gene sequencing

Cell cycles and their regulation.

Cell death—events, regulators, intrinsic and extrinsic pathways of apoptosis.

Necrosis and autophagy.

Unit-2: Cell Signaling                                                                                                    (12 hours)

Intercellular and intracellular signaling pathways.

Classification of receptor family and molecular structure: ligand-gated ion channels, G-protein coupled receptors, tyrosine kinase receptors, and nuclear receptors.

Secondary Messengers: cyclic AMP, cyclic GMP, calcium ion, inositol 1,4,5-trisphosphate (IP3), NO, and diacylglycerol.

Detailed study of the following intracellular signaling pathways: cyclic AMP signaling pathway, mitogen-activated protein kinase (MAPK) signaling, and Janus kinase (JAK)/signal transducer and activator of transcription (STAT) signaling pathway.

Unit-3: Principles and Applications of Genomic and Proteomic Tools            (12 hours)

DNA electrophoresis, PCR (reverse transcription and real time), gene sequencing, microarray technique, SDS page, ELISA, and western blotting. Recombinant DNA technology and gene therapy

Basic principles of recombinant DNA technology—restriction enzymes and various types of vectors. Applications of recombinant DNA technology.

Gene therapy: various types of gene transfer techniques, clinical applications, and recent advances in gene therapy.

Unit-4: Pharmacogenomics                                                                                          (12 hours)

Gene mapping and cloning of disease genes.

Genetic variation and its role in health/pharmacology

Polymorphisms affecting drug metabolism

Genetic variation in drug transporters

Genetic variation in G protein-coupled receptors

Applications of Proteomics Science: Genomics, proteomics, metabolomics, functionomics, nutrigenomics

Immunotherapeutics

Types of immunotherapeutics, humanisation antibody therapy, Immunotherapeutics in clinical practice

Unit-5:                                                                                                                                (12 hours)

a. Cell Culture Techniques

Basic equipment used in a cell culture lab. Cell culture media, various types of cell culture, general procedure for cell cultures, isolation of cells, subculture, cryopreservation, characterization of cells, and their application.

Principles and applications of cell viability assays, glucose uptake assays, and Calcium influx assays

Principles and applications of flow cytometry

b. Biosimilars