Instrumental Methods of Analysis

Syllabus

 Module 01                                                                                                                        

UV Visible Spectroscopy

• Electronic transitions, chromophores, auxochromes, spectral shifts, solvent effect on absorption spectra, Beer and Lambert’s law, Derivation and deviations.
• Instrumentation - Sources of radiation, wavelength selectors, sample cells, detectors-Photo tube, Photomultiplier tube, Photo voltaic cell, Silicon Photodiode.
• Applications - Spectrophotometric titrations, Single component and multi component analysis.

Fluorimetry

• Theory, Concepts of singlet, doublet and triplet electronic states, internal and external conversions, factors affecting fluorescence, quenching, instrumentation and applications. 

Module 02                                                                                                                         

IR spectroscopy

• Introduction, fundamental modes of vibrations in poly atomic molecules, sample

handling, factors affecting vibrations.

• Instrumentation - Sources of radiation, wavelength selectors, detectors - Golay cell, Bolometer, Thermocouple, Thermister, Pyroelectric detector and applications.

Flame Photometry

• Principle, interferences, instrumentation and applications.

Atomic Absorption Spectroscopy

• Principle, interferences, instrumentation and applications.

Nepheloturbidometry

• Principle, instrumentation and applications.

Module 03                                                                                                                       

Introduction to chromatography

Adsorption and partition column chromatography

• Methodology, advantages, disadvantages and applications.

Thin layer chromatography

• Introduction, Principle, Methodology, R f va lu es, advantages, disadvantages and

applications.

Paper Chromatography

• Introduction, methodology, development techniques, advantages, disadvantages and applications.

Electrophoresis

•  Introduction, factors affecting electrophoretic mobility, Techniques of paper, gel,

capillary electrophoresis, applications.

Module 04                                                                                                                        

Gas Chromatography

• Introduction, theory, instrumentation, derivatization, temperature programming,

advantages, disadvantages and applications.

High Performance Liquid Chromatography (HPLC)

• Introduction, theory, instrumentation, advantages and applications.

Module 05                                                                                                                          

Ion exchange chromatography

• Introduction, classification, ion exchange resins, properties, mechanism of ion

exchange process, factors affecting ion exchange, methodology and applications.

Gel chromatography

• Introduction, theory, instrumentation and applications.

Affinity chromatography

• Introduction, theory, instrumentation and applications.