Gene Technology, Immunology and Computational Biology (Zoology)

Unit

Topic

Total No. of

Lectures (60)

I

Principles of Gene Manipulation

· Recombinant DNA Technology

· Selection and identification of recombinant cells

· Restriction enzymes, DNA modifying enzymes, Cloning Vectors, Ligation

·  Gene transfer techniques, Gene therapy

10

II

Applications of Genetic Engineering

· Single cell proteins

· Biosensors, Biochips

· Crop and live stock improvement, Development of transgenics

· Development of DNA drugs and vaccines

8

III

DNA Diagnostics

· Genetic analysis of human diseases, Detection of known and unknown mutations

· Concept of pharmacogenomics and pharmacogenetics

4

IV

Immune System and its Components

· Historical perspective of Immunology, Innate and Adaptive Immunity, Clonal selection, Complement system

· Structure and functions of different classes of immunoglobulins, Hypersensitivity

· Humoral immunity and cell mediated immunity

· HLA complex: Organisation, class I and II HLA molecules

10

V

Biostatistics I

· Calculations of mean, median, mode, variance, standard deviation

· Concepts of coefficient of variation, Skewness, Kurtosis

· Elementary idea of probability and application

7

VI

Biostatistics II

· Data summarizing: Frequency distribution, Graphical presentation—Bar, Pie diagram, Histogram

· Tests of significance: One and two sample tests, t-test and Chisquare test

7

VII

Basics of Computers

· Basics (CPU, I/O units) and Operating systems

· Concept of homepages and websites, World Wide Web, URLs, using search engines

6

VIII

Bioinformatics

· Databases: Nucleic acids, Genomes, Protein sequences and structures, Bibliography

· Sequence analysis (homology): Pairwise and multiple sequence alignments-Blast, Clustalw

· Phylogenetic analysis

8