Pharmaceutical Biotechnology

Syllabus

Module 01                                                                                       10 Hours

• Brief introduction to Biotechnology with reference to Pharmaceutical Sciences.
• Enzyme Biotechnology- Methods of enzyme immobilization and applications.
• Biosensors- Working and applications of biosensors in Pharmaceutical Industries.
• Brief introduction to Protein Engineering.
• Use of microbes in industry. Production of Enzymes- General consideration - Amylase, Catalase, Peroxidase, Lipase, Protease, Penicillinase.
• Basic principles of genetic engineering.

Module 02                                                                                        10 Hours

• Study of cloning vectors, restriction endonucleases and DNA ligase.
• Recombinant DNA technology. Application of genetic engineering in medicine.
• Application of rDNA technology and genetic engineering in the production of: i)
• Interferon ii) Vaccines- hepatitis- B iii) Hormones-Insulin.
• Brief introduction to PCR.

Module 03                                                                                        10 Hours

• Types of immunity- humoral immunity, cellular immunity.
• Structure of Immunoglobulins.
• Structure and Function of MHC.
• Hypersensitivity reactions, Immune stimulation and Immune suppressions.
• General method of the preparation of bacterial vaccines, toxoids, viral vaccine, antitoxins, serum-immune blood derivatives and other products relative to immunity.
• Storage conditions and stability of official vaccines.
• Hybridoma technology- Production, Purification and Applications.
• Blood products and Plasma Substitutes.

Module 04                                                                                        08 Hours

Immuno-Blotting Techniques

• ELISA, Western blotting, Southern blotting.
• Genetic organization of Eukaryotes and Prokaryotes.
• Microbial genetics including transformation, transduction, conjugation, plasmids and transposons.
• Introduction to Microbial biotransformation and applications.

Mutation

Types of mutation/mutants.

Module 05                                                                                        07 Hours

• Fermentation methods and general requirements, study of media, equipments, sterilization methods, aeration process, stirring.
• Large-scale production fermenter design and its various controls.
• Study of the production of - penicillins, citric acid, Vitamin B12, Glutamic acid, Griseofulvin.

Blood Products

• Collection, Processing and Storage of whole human blood, dried human plasma, plasma Substituties.

• Recombinant DNA technology. Application of genetic engineering in medicine.
• Application of rDNA technology and genetic engineering in the production of: i)
• Interferon ii) Vaccines- hepatitis- B iii) Hormones-Insulin.
• Brief introduction to PCR.