Instrumental Methods of Analysis

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ISBN No.- 978-93-90031-65-8

Dr. S. Malathi

Dr. Pallavi Mangesh Patil

Dr. Sunil Kumar

Syllabus

 Module 01                                                                                                                        

UV Visible Spectroscopy

  • Electronic transitions, chromophores, auxochromes, spectral shifts, solvent effect on absorption spectra, Beer and Lambert’s law, Derivation and deviations.
  • Instrumentation - Sources of radiation, wavelength selectors, sample cells, detectors-Photo tube, Photomultiplier tube, Photo voltaic cell, Silicon Photodiode.
  • Applications - Spectrophotometric titrations, Single component and multi component analysis.

 

Fluorimetry

  • Theory, Concepts of singlet, doublet and triplet electronic states, internal and external conversions, factors affecting fluorescence, quenching, instrumentation and applications. 

 

Module 02                                                                                                                         

IR spectroscopy

  • Introduction, fundamental modes of vibrations in poly atomic molecules, sample

handling, factors affecting vibrations.

  • Instrumentation - Sources of radiation, wavelength selectors, detectors - Golay cell, Bolometer, Thermocouple, Thermister, Pyroelectric detector and applications.

 

Flame Photometry

  • Principle, interferences, instrumentation and applications.

 

Atomic Absorption Spectroscopy

  • Principle, interferences, instrumentation and applications.

 

Nepheloturbidometry

  • Principle, instrumentation and applications.

 

Module 03                                                                                                                       

Introduction to chromatography

Adsorption and partition column chromatography

  • Methodology, advantages, disadvantages and applications.

 

Thin layer chromatography

  • Introduction, Principle, Methodology, R f va lu es, advantages, disadvantages and

applications.

 

Paper Chromatography

  • Introduction, methodology, development techniques, advantages, disadvantages and applications.

 

Electrophoresis

  •  Introduction, factors affecting electrophoretic mobility, Techniques of paper, gel,

capillary electrophoresis, applications.

Module 04                                                                                                                        

Gas Chromatography

  • Introduction, theory, instrumentation, derivatization, temperature programming,

advantages, disadvantages and applications.

 

High Performance Liquid Chromatography (HPLC)

  • Introduction, theory, instrumentation, advantages and applications.

 

Module 05                                                                                                                          

Ion exchange chromatography

  • Introduction, classification, ion exchange resins, properties, mechanism of ion

exchange process, factors affecting ion exchange, methodology and applications.

 

Gel chromatography

  • Introduction, theory, instrumentation and applications.

 

Affinity chromatography

  • Introduction, theory, instrumentation and applications.
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