Instrumental Methods of Analysis

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The Instrumental Methods of Analysis book is an essential guide for B.Pharm 7th semester students. This comprehensive book covers essential topics such as chromatography, spectroscopy, and electrophoresis.

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With practical examples and case studies, this book is perfect for students looking to excel in the field of pharmaceutical analysis. Order your copy today and enhance your knowledge!

Dr. S. Malathi, Dr. Pallavi Mangesh Patil, Dr. Sunil Kumar

ISBN No.: 978-93-90031-65-8

Syllabus

 Module 01                                                                                                                        

UV Visible Spectroscopy

  • Electronic transitions, chromophores, auxochromes, spectral shifts, solvent effect on absorption spectra, Beer and Lambert’s law, Derivation and deviations.
  • Instrumentation - Sources of radiation, wavelength selectors, sample cells, detectors-Photo tube, Photomultiplier tube, Photo voltaic cell, Silicon Photodiode.
  • Applications - Spectrophotometric titrations, Single component and multi component analysis.

 

Fluorimetry

  • Theory, Concepts of singlet, doublet and triplet electronic states, internal and external conversions, factors affecting fluorescence, quenching, instrumentation and applications. 

 

Module 02                                                                                                                         

IR spectroscopy

  • Introduction, fundamental modes of vibrations in poly atomic molecules, sample

handling, factors affecting vibrations.

  • Instrumentation - Sources of radiation, wavelength selectors, detectors - Golay cell, Bolometer, Thermocouple, Thermister, Pyroelectric detector and applications.

 

Flame Photometry

  • Principle, interferences, instrumentation and applications.

 

Atomic Absorption Spectroscopy

  • Principle, interferences, instrumentation and applications.

 

Nepheloturbidometry

  • Principle, instrumentation and applications.

 

Module 03                                                                                                                       

Introduction to chromatography

Adsorption and partition column chromatography

  • Methodology, advantages, disadvantages and applications.

 

Thin layer chromatography

  • Introduction, Principle, Methodology, R f va lu es, advantages, disadvantages and

applications.

 

Paper Chromatography

  • Introduction, methodology, development techniques, advantages, disadvantages and applications.

 

Electrophoresis

  •  Introduction, factors affecting electrophoretic mobility, Techniques of paper, gel,

capillary electrophoresis, applications.

Module 04                                                                                                                        

Gas Chromatography

  • Introduction, theory, instrumentation, derivatization, temperature programming,

advantages, disadvantages and applications.

 

High Performance Liquid Chromatography (HPLC)

  • Introduction, theory, instrumentation, advantages and applications.

 

Module 05                                                                                                                          

Ion exchange chromatography

  • Introduction, classification, ion exchange resins, properties, mechanism of ion

exchange process, factors affecting ion exchange, methodology and applications.

 

Gel chromatography

  • Introduction, theory, instrumentation and applications.

 

Affinity chromatography

  • Introduction, theory, instrumentation and applications.

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